Microwave energy is a fast, uniform and effective method for pre-treatments of textile materials. Using pectinase, cellulase, laccase, etc. enzymes for treatments of cotton textile material before colouration have many advantages over conventional methods such as reducing the chemical damage of fibers, short process time, energy and water savings. Recently, microwave-assisted enzymatic treatment is a sustainable cleaner production technique for all natural fibers. Microwave-assisted dyeing and finishing methods can be applied successfully in the literature. There are limited works about microwave-assisted enzymatic treatment of cotton. In this work, the effect of microwave-assisted treatments with NaOH, pectinase, cellulase, laccase and combination of pectinase/cellulase, pectinase/laccase, pectinase/cellulase/laccase, pectinase/laccase/cellulase enzymes on conventional reactive dyeability of cotton fabric. Hydrophilicity, contact angle, whiteness/yellowness indexes of conventionally and microwave-assisted treated samples were measured and compared with conventionally treated samples with sodium hydroxide. Whiteness and yellowness results were adequate for dark-coloured samples. The CIELab colour coordinates, colour differences and fastness tests to rubbing/washing results of dyed samples were examined. The pilling test results (4+) and colour fastness to rubbing/washing (4-5 +) were investigated and adequate results were obtained. The time savings was almost 90% after microwave-assisted treatments. Finally, microwave-assisted enzymatic treatments can be an environmental and sustainable method due to short processing times. In addition using microwave-assisted enzymatic treatment processes were contributed to reduction of carbon footprint.