Fiber based scaffolds for tissue engineering applications manufactured through textile technologies feature an anisotropic, highly porous, and controllable macro-, micro-, and nanostructure. For the fabrication of fiber based collagen scaffolds, collagen multifilament yarns are required. The paper summarizes a scalable collagen multifilament yarn production method. Collagen yarn production was realized via wet spinning and a 3D printed spin packs comprised of coaxial nozzles. Mechanical, thermal, structural, and biological characteristics of wet-spun collagen yarns are given.