The fibrous scaffold production with desired structure plays an important role in multidisciplinary field of tissue engineering. Electrospinning is popular approach for creating nanofibrous substrates in which the filaments resembling the natural extra cellular matrix (ECM) can provide topographical cues to cells directing their growth. One of the major challenges in electrospinning is tailoring the spatial organization of the filaments. To overcome this challenge, a hybrid static collector was utilized that enabled us to form distinct filament organizations on a single substrate. The effect of the filament organization on cellular growth was studied. The engineered scaffolds are promising candidates for fabrication interfaces between various tissues.