Skin injury from burns is an awful condition that leads to an increase of fluid loss, hypothermia, scarring and infections. Despite advances in therapy, infections remain a major cause of morbidity and mortality in burn patients. In recent years, wound dressing has developed according to the pathogenesis of different wound. Different types of wound dressing including xerogels, charcoal cloth, alginates, chitosan, and hydrogels have been introduced for this purpose through the last decades. Recently, many researchers have focused on the use of biological materials like chitin and chitosan. Chitosan is a versatile biopolymer rendering it a variety of applications including wound dressing film. However, in a form of film, chitosan possesses poor mechanical properties including tear strength and handle ability. For this reason, chitosan/acrylic laminate pad was prepared, aiming at the wound dressing pad with antibacterial performance. In this work, we prepared chitosan/acrylic laminate pad using a casting method. Chitosan solution was prepared by dissolving chitosan 1% (w/v) in 1% (v/v) acetic acid solution. Then, chitosan solution was poured onto a precast acrylic film (prepared from acrylic emulsion) and allowed to completely dry at room temperature for a couple of days to achieve transparent and flexible laminate film. This film exhibited fast water absorption without loss of mechanical properties. Based on this property, the chitosan/acrylic laminate film is potential for wound dressing application. Antibacterial activity against Staphylococcus aureus (ATCC 6538P) of samples was carried out by disk diffusion method on an agar plate. Diameter of the zone of inhibition was measured as per the standard test procedure. The antibacterial activity of the chitosan/acrylic laminate film against S. aureus was achieved as expected.